pGBKT7-RmMYB108
(Plasmid
#173183)
-
PurposeExpresses Rosa multiflora MYB108 for transcriptional activation studies in yeast.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 173183 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGBKT7
-
Vector typeYeast Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsFor yeast expression, use Yeast AH109 strain and grow at 30 degrees C.
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameRmMYB108
-
SpeciesRosa multiflora
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGBKT7-RmMYB108 was a gift from Daidi Che (Addgene plasmid # 173183 ; http://n2t.net/addgene:173183 ; RRID:Addgene_173183) -
For your References section:
An R2R3-MYB Transcription Factor RmMYB108 Responds to Chilling Stress of Rosa multiflora and Conferred Cold Tolerance of Arabidopsis. Dong J, Cao L, Zhang X, Zhang W, Yang T, Zhang J, Che D. Front Plant Sci. 2021 Jul 27;12:696919. doi: 10.3389/fpls.2021.696919. eCollection 2021. 10.3389/fpls.2021.696919 PubMed 34386027