pIRES2_EGFP_KCNQ1
(Plasmid #173161)

• Purpose: For mammalian expression of KCNQ1 and creating variants
• Depositing Lab: Al George
• Publication: Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e002345. doi: 10.1161/CIRCGEN.118.002345.

Backbone

• Vector backbone: pIRES2_EGFP
• Backbone size w/o insert (bp): 5519
• Total vector size (bp): 7548
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KCNQ1
• Alt name: Kv7.1
• Species: H. sapiens (human)
• Insert Size (bp): 2029
• Entrez Gene: KCNQ1 (a.k.a. ATFB1, ATFB3, JLNS1, KCNA8, KCNA9, KVLQT1, Kv1.9, Kv7.1, LQT, LQT1, RWS, SQT2, WRS)
• Promoter: CMV
• Tag / Fusion Protein:
  • IRES2-EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: V1-5
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry