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Addgene

pIRES_EGFP_GA_KCNE1
(Plasmid #173160)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173160 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES_EGFP
  • Backbone size w/o insert (bp) 5306
  • Total vector size (bp) 5696
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KCNE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    390
  • Entrez Gene
    KCNE1 (a.k.a. ISK, JLNS, JLNS2, LQT2/5, LQT5, MinK)
  • Promoter CMV
  • Tag / Fusion Protein
    • IRES2-EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer IRES2F
  • 3′ sequencing primer IRES2R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES_EGFP_GA_KCNE1 was a gift from Al George (Addgene plasmid # 173160 ; http://n2t.net/addgene:173160 ; RRID:Addgene_173160)
  • For your References section:

    Allosteric mechanism for KCNE1 modulation of KCNQ1 potassium channel activation. Kuenze G, Vanoye CG, Desai RR, Adusumilli S, Brewer KR, Woods H, McDonald EF, Sanders CR, George AL Jr, Meiler J. Elife. 2020 Oct 23;9. pii: 57680. doi: 10.7554/eLife.57680. 10.7554/eLife.57680 PubMed 33095155