pIRES_EGFP_GA_KCNE1
(Plasmid
#173160)
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PurposeFor mammalian expression of KCNE1 and creating variants
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173160 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRES_EGFP
- Backbone size w/o insert (bp) 5306
- Total vector size (bp) 5696
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKCNE1
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SpeciesH. sapiens (human)
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Insert Size (bp)390
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Entrez GeneKCNE1 (a.k.a. ISK, JLNS, JLNS2, LQT2/5, LQT5, MinK)
- Promoter CMV
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Tag
/ Fusion Protein
- IRES2-EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer IRES2F
- 3′ sequencing primer IRES2R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRES_EGFP_GA_KCNE1 was a gift from Al George (Addgene plasmid # 173160 ; http://n2t.net/addgene:173160 ; RRID:Addgene_173160) -
For your References section:
Allosteric mechanism for KCNE1 modulation of KCNQ1 potassium channel activation. Kuenze G, Vanoye CG, Desai RR, Adusumilli S, Brewer KR, Woods H, McDonald EF, Sanders CR, George AL Jr, Meiler J. Elife. 2020 Oct 23;9. pii: 57680. doi: 10.7554/eLife.57680. 10.7554/eLife.57680 PubMed 33095155
