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pCS2_IRES2_CyOFP1_KCNQ2 var4
(Plasmid #173155)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173155 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2_IRES2_CyOFP1
  • Backbone size w/o insert (bp) 5671
  • Total vector size (bp) 8197
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KCNQ2
  • Alt name
    Kv7.2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2526
  • Entrez Gene
    KCNQ2 (a.k.a. BFNC, DEE7, EBN, EBN1, ENB1, HNSPC, KCNA11, KV7.2)
  • Promoter Simian CMV IE94
  • Tag / Fusion Protein
    • IRES2-CyOFP1 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV_F
  • 3′ sequencing primer SV40pA_R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2_IRES2_CyOFP1_KCNQ2 var4 was a gift from Al George (Addgene plasmid # 173155 ; http://n2t.net/addgene:173155 ; RRID:Addgene_173155)

  • For your References section:

    Dyshomeostatic modulation of Ca(2+)-activated K(+) channels in a human neuronal model of KCNQ2 encephalopathy. Simkin D, Marshall KA, Vanoye CG, Desai RR, Bustos BI, Piyevsky BN, Ortega JA, Forrest M, Robertson GL, Penzes P, Laux LC, Lubbe SJ, Millichap JJ, George AL Jr, Kiskinis E. Elife. 2021 Feb 5;10. pii: 64434. doi: 10.7554/eLife.64434. 10.7554/eLife.64434 PubMed 33544076
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