pcDNA3.1(+)-C-6His-mEsrra
(Plasmid
#173152)
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Purposemouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173152 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)-C-6His
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Backbone manufacturerGenescript
- Backbone size w/o insert (bp) 5432
- Total vector size (bp) 6710
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Modifications to backbonenone
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameORF clone Estrogen-related receptor alpha (Esrra)
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Alt nameERRa
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Alt nameEsrra
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SpeciesM. musculus (mouse)
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Insert Size (bp)1278
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MutationWild type
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GenBank IDNM_007953.2 NM_007953.2
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Entrez GeneEsrra (a.k.a. ERRalpha, Err1, Estrra, Nr3b1)
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer TAATACGACTCACTATAGG (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCustom generated by Genescript upon order.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)-C-6His-mEsrra was a gift from Brijesh Kumar Singh (Addgene plasmid # 173152 ; http://n2t.net/addgene:173152 ; RRID:Addgene_173152) -
For your References section:
Esrra regulates Rplp1-mediated translation of lysosome proteins suppressed in non-alcoholic steatohepatitis and reversed by alternate day fasting. Tripathi M, Gauthier K, Sandireddy R, Zhou J, Gupta P, Sakthivel S, Teo WW, Naing YT, Arul K, Tikno K, Park S-H, Wu Y, Wang L, Bay B-H, Sun L, Giguere V, Chow PKH, Ghosh S, McDonnell DP, Yen PM, Singh BK. bioRxiv 2021.11.16.468891 10.1101/2021.11.16.468891