pDONR221-kcnj20
(Plasmid #173150)

• Purpose: This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
• Depositing Lab: GuangJun Zhang
• Publication: Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.425.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2550
• Total vector size (bp): 3735
• Vector type: PCR cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: kcnj20
• Alt name: si:ch211-113j13.2
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1185
• GenBank ID: XM_021466526.1
• Entrez Gene: si:ch211-113j13.2
• Promoter: No promoter at 5 end; T7 promoter at 3 end.

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.

The DNA sequences were amplified from pooled wild type TAB zebrafish embryos. R54C (compared to XM_021466526.1) is likely a polymorphisms, which are not listed in the current ENSEMBL database. This is possibly due to incomplete zebrafish genome annotation, GRCz11. However, this will not affect the result of whole-mount in situ hybridization