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PurposeExpresses pheS_A294G by dual T7 promoter in e.coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173147 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVS133
- Backbone size w/o insert (bp) 9875
- Total vector size (bp) 10919
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepheS_A294G
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SpeciesSynthetic
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer 5'-CACTATAGGGGAATTGTGAGC-3'
- 3′ sequencing primer 5'-GGTTATGCTAGTTATTGCTCAGC-3' (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHyo245 was a gift from Seokhee Kim (Addgene plasmid # 173147 ; http://n2t.net/addgene:173147 ; RRID:Addgene_173147) -
For your References section:
Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo. Park H, Kim S. Nucleic Acids Res. 2021 Apr 6;49(6):e32. doi: 10.1093/nar/gkaa1231. 10.1093/nar/gkaa1231 PubMed 33406230
Map uploaded by the depositor.
