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pDONR221-kcnj11
(Plasmid #173139)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2550
  • Total vector size (bp) 3696
  • Vector type
    PCR cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kcnj11
  • Alt name
    Kir6.2
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1146
  • GenBank ID
    NM_001039827.1
  • Entrez Gene
    kcnj11 (a.k.a. Kir6, kir6.2)
  • Promoter No promoter at 5 end; T7 promoter at 3 end.

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-kcnj11 was a gift from GuangJun Zhang (Addgene plasmid # 173139 ; http://n2t.net/addgene:173139 ; RRID:Addgene_173139)

  • For your References section:

    Evolution of inwardly rectifying potassium channels and their gene expression in zebrafish embryos. Silic MR, Murata SH, Park SJ, Zhang G. Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.425. 10.1002/dvdy.425 PubMed 34558132
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