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Addgene

pHyo182
(Plasmid #173136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173136 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVS133
  • Backbone size w/o insert (bp) 9794
  • Total vector size (bp) 10838
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pheS_A294G
  • Species
    Synthetic

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer T7promoter(5'-TAATACGACTCACTATAGGG-3')
  • 3′ sequencing primer T7terminator(5'-GCTAGTTATTGCTCAGCGG-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHyo182 was a gift from Seokhee Kim (Addgene plasmid # 173136 ; http://n2t.net/addgene:173136 ; RRID:Addgene_173136)

  • For your References section:

    Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo. Park H, Kim S. Nucleic Acids Res. 2021 Apr 6;49(6):e32. doi: 10.1093/nar/gkaa1231. 10.1093/nar/gkaa1231 PubMed 33406230
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