MDO1-c014
(Plasmid
#173103)
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PurposeExpression of recombinant fusion protein in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 173103 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNIC-CFP
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMACROD1
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SpeciesH. sapiens (human)
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MutationS58-A325, codon optimized
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Entrez GeneMACROD1 (a.k.a. LRP16)
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Tag
/ Fusion Protein
- mCerulean (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.05.31.445082v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MDO1-c014 was a gift from Lari Lehtiö (Addgene plasmid # 173103 ; http://n2t.net/addgene:173103 ; RRID:Addgene_173103) -
For your References section:
A molecular toolbox for ADP-ribosyl binding proteins. Sowa ST, Galera-Prat A, Wazir S, Alanen HI, Maksimainen MM, Lehtio L. Cell Rep Methods. 2021 Dec 20;1(8):100121. doi: 10.1016/j.crmeth.2021.100121. Epub 2021 Nov 11. 10.1016/j.crmeth.2021.100121 PubMed 34786571