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Addgene

PARP15-c021
(Plasmid #173102)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173102 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC-CFP
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PARP15
  • Species
    H. sapiens (human)
  • Mutation
    G287-N470, codon optimized
  • Entrez Gene
    PARP15 (a.k.a. ARTD7, BAL3, pART7)
  • Tag / Fusion Protein
    • mCerulean (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PARP15-c021 was a gift from Lari Lehtiö (Addgene plasmid # 173102 ; http://n2t.net/addgene:173102 ; RRID:Addgene_173102)

  • For your References section:

    A molecular toolbox for ADP-ribosyl binding proteins. Sowa ST, Galera-Prat A, Wazir S, Alanen HI, Maksimainen MM, Lehtio L. Cell Rep Methods. 2021 Dec 20;1(8):100121. doi: 10.1016/j.crmeth.2021.100121. Epub 2021 Nov 11. 10.1016/j.crmeth.2021.100121 PubMed 34786571
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