pNH-NanoLuc
(Plasmid
#173075)
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Purpose(Empty Backbone) Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173075 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNH-TrxT (but TrxT removed)
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Backbone manufacturerOpher Gileadi (Addgene plasmid 26106)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pNH-TrxT that contains the sequence of Nanoluciferase in place of TrxT before the TEV cleavage site. Please visit https://www.biorxiv.org/content/10.1101/2021.05.31.445082v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNH-NanoLuc was a gift from Lari Lehtiö (Addgene plasmid # 173075 ; http://n2t.net/addgene:173075 ; RRID:Addgene_173075) -
For your References section:
A molecular toolbox for ADP-ribosyl binding proteins. Sowa ST, Galera-Prat A, Wazir S, Alanen HI, Maksimainen MM, Lehtio L. Cell Rep Methods. 2021 Dec 20;1(8):100121. doi: 10.1016/j.crmeth.2021.100121. Epub 2021 Nov 11. 10.1016/j.crmeth.2021.100121 PubMed 34786571
