pCS2-mCherry_SNAP_SNAPV_PACT_Dead
(Plasmid
#173073)
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PurposeDead SNAP tag at the centrosome
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4091
- Total vector size (bp) 6686
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry-SNAP-SNAP-Varied-PACT
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Alt namepericentrin AKAP450 centrosomal-targeting domain
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SpeciesH. sapiens (human)
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Insert Size (bp)2627
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MutationAA144 in both SNAP tags changed from a cysteine to an alanine
- Promoter CMV IE94
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Tag
/ Fusion Protein
- mCherry-SNAP-SNAP-Varied (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJohn Scott Lab, University of Washington, Seattle WA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The SNAP gene/insert was a gift from the Scott lab as part of collaboration on the manuscript entitled "Subcellular drug targeting illuminates local kinase action"
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-mCherry_SNAP_SNAPV_PACT_Dead was a gift from Heidi Hehnly (Addgene plasmid # 173073 ; http://n2t.net/addgene:173073 ; RRID:Addgene_173073) -
For your References section:
Subcellular drug targeting illuminates local kinase action. Bucko PJ, Lombard CK, Rathbun L, Garcia I, Bhat A, Wordeman L, Smith FD, Maly DJ, Hehnly H, Scott JD. Elife. 2019 Dec 24;8. pii: 52220. doi: 10.7554/eLife.52220. 10.7554/eLife.52220 PubMed 31872801