hSYN-mito-dsRED2
(Plasmid #173069)

• Purpose: mitochondria targeted dsRED2 expressed off of human Synapsin I promoter.
• Depositing Lab: Thomas Schwarz
• Publication: Fluorescent plasmids for live cell imaging in primary neurons (unpublished)

Backbone

• Vector backbone: pLVX
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 6837
• Total vector size (bp): 7623
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mito
• Alt name: COX8A presequence
• Species: H. sapiens (human)
• Insert Size (bp): 75
• GenBank ID: NM_004074.3 NM_004074.3
• Promoter: human synapsin 1
• Tag / Fusion Protein:
  • dsRED (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: hSyn promoter was cloned from addgene plasmid 22909, mito-dsRED was cloned from addgene plasmid 55838.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry