Arabidopsis thaliana Atm3 d80 pET21a+
(Plasmid
#173045)
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PurposeExpresses AtAtm3 in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173045 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21a(+)
- Backbone size w/o insert (bp) 5340
- Total vector size (bp) 7320
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameArabidopsis thaliana Atm3
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1980
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Mutationwith N-terminal 80 amino acids deletion
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GenBank IDNM_125212.3
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Entrez GeneABCB25 (a.k.a. AT5G58270, ABC transporter of the mitochondrion 3, ARABIDOPSIS THALIANA ABC TRANSPORTER OF THE MITOCHONDRION 3, ATATM3, ATM3, ATP-binding cassette B25, HALF-MOLECULE ABC TRANSPORTER ATM3, MCK7.14, MCK7_14, STA1, STARIK 1)
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Tag
/ Fusion Protein
- 6xHis tag (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byOrdered from GenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Arabidopsis thaliana Atm3 d80 pET21a+ was a gift from Douglas Rees (Addgene plasmid # 173045 ; http://n2t.net/addgene:173045 ; RRID:Addgene_173045) -
For your References section:
Glutathione binding to the plant AtAtm3 transporter and implications for the conformational coupling of ABC transporters. Fan C, Rees DC. Elife. 2022 Mar 25;11. pii: 76140. doi: 10.7554/eLife.76140. 10.7554/eLife.76140 PubMed 35333177