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Addgene

AAV-Camk2-NES-Twitch-NR
(Plasmid #173043)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173043 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Lentivirus
  • Backbone size w/o insert (bp) 9245
  • Total vector size (bp) 10937
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NES-Twitch-NR
  • Insert Size (bp)
    1692
  • Promoter CamK2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GTCAAGCCGGTTCTCCGTTTGCACTC
  • 3′ sequencing primer CAGCGTATCCACATAGCGTAAAAGGAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-Camk2-NES-Twitch-NR was a gift from Yiyang Gong (Addgene plasmid # 173043 ; http://n2t.net/addgene:173043 ; RRID:Addgene_173043)

  • For your References section:

    Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins. Zhang D, Redington E, Gong Y. Commun Biol. 2021 Jul 29;4(1):924. doi: 10.1038/s42003-021-02452-z. 10.1038/s42003-021-02452-z PubMed 34326458
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