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Addgene

pRMCD77
(Plasmid #173032)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173032 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRMCD77
  • Backbone size (bp) 3300
  • Modifications to backbone
    pET17b was digested with BglII and end-filled with dNTPs using Klenow followed by re-ligation to form pRMCD77.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRMCD77 was a gift from Renato Morona (Addgene plasmid # 173032 ; http://n2t.net/addgene:173032 ; RRID:Addgene_173032)

  • For your References section:

    Analysis of Shigella flexneri wzz (Rol) function by mutagenesis and cross-linking: wzz is able to oligomerize. Daniels C, Morona R. Mol Microbiol. 1999 Oct;34(1):181-94. doi: 10.1046/j.1365-2958.1999.01591.x. 10.1046/j.1365-2958.1999.01591.x PubMed 10540296
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