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pCMV-PV-NLS-CDEF-GFP
(Plasmid #17303)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17303 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV-Myc-Cyto-GFP
  • Backbone size w/o insert (bp) 5900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PV-NLS-CDEF-GFP
  • Alt name
    Parvalbumin
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1100
  • Mutation
    Functional Ca2+ binding sites (CD or EF Domain ) were inactivated by substituting a glutamate for a valine residue at position 12 of each Ca2 + binding loop.
  • GenBank ID
    M12725
  • Entrez Gene
    Pvalb (a.k.a. PALB1, Pva)
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SaII (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer pCMV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-PV-NLS-CDEF-GFP was a gift from Anton Bennett (Addgene plasmid # 17303)

  • For your References section:

    Epidermal growth factor-mediated activation of the ETS domain transcription factor Elk-1 requires nuclear calcium. Pusl T, Wu JJ, Zimmerman TL, Zhang L, Ehrlich BE, Berchtold MW, Hoek JB, Karpen SJ, Nathanson MH, Bennett AM. J Biol Chem. 2002 Jul 26. 277(30):27517-27. 10.1074/jbc.M203002200 PubMed 11971908
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