EZ-L545
(Plasmid
#173024)
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PurposeEncodes OptoEXP2 circuit
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173024 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVP16-EL222
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SpeciesErythrobacter litoralis
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Insert Size (bp)900
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Tag
/ Fusion Protein
- SV40 NLS (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byKevin Gardner and Laura Motta-Mena
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EZ-L545 was a gift from Jose Avalos (Addgene plasmid # 173024 ; http://n2t.net/addgene:173024 ; RRID:Addgene_173024) -
For your References section:
Optogenetic Amplification Circuits for Light-Induced Metabolic Control. Zhao EM, Lalwani MA, Chen JM, Orillac P, Toettcher JE, Avalos JL. ACS Synth Biol. 2021 May 21;10(5):1143-1154. doi: 10.1021/acssynbio.0c00642. Epub 2021 Apr 9. 10.1021/acssynbio.0c00642 PubMed 33835777