pcDNA3-Lyn-GR-AKAR3
(Plasmid #173016)

• Purpose: Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.
• Depositing Lab: Jin Zhang
• Publication: Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi: 10.1038/s41467-020-15687-x.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5414
• Total vector size (bp): 7694
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lyn-GR-AKAR3
• Insert Size (bp): 2280
• Promoter: CMV
• Tags / Fusion Proteins:
  • N-terminal targeting sequence from Lyn kinase (N terminal on insert)
  • EGFP (N terminal on insert)
  • StagRFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-Lyn-GR-AKAR3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry