DHFR-GFP-th-GluA1
(Plasmid #173008)

• Purpose: For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.
• Depositing Lab: Matthew Kennedy
• Publication: Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212461. doi: 10.1083/jcb.202103186. Epub 2021 Jul 9.

Backbone

• Vector backbone: pLenti
• Backbone size w/o insert (bp): 8420
• Total vector size (bp): 12425
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DHFR-GFP-th-GluA1
• Species: R. norvegicus (rat)
• Insert Size (bp): 4005
• Promoter: human synapsin
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gaccatctgcgctgcgg
• 3′ sequencing primer: agcgtatccacatagcgt

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GluA1 ORF was a gift from Dr. Michael Ehlers

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.09.02.278770v1 for bioRxiv preprint.