pLionII
(Plasmid #1730)

• Purpose: (Empty Backbone) This is a 3rd generation lentiviral transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system
• Depositing Lab: Garry Nolan
• Publication: Nolan Lab Plasmids (unpublished)

Backbone

• Vector backbone: pLionII
• Backbone manufacturer: Nolan Lab
• Backbone size (bp): 7002
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: M13 reverse primer

Resource Information

• Supplemental Documents:
  • Nolan-felix
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Nolan plasmid Ancillary Agreement
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system.
The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB.

Please note that the AflII site is not unique, as suggested by the plasmid map and full sequence information.