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Addgene

MAC-N-GFP
(Plasmid #172974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MAC-tag-C
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Alt name
    Green fluorescent protein
  • Species
    Aequorea victoria
  • GenBank ID
    AAA27722.1
  • Tag / Fusion Protein
    • MAC-tag (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: this plasmid contains a Y238F mutation in GFP. This mutation does not affect the plasmid's function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAC-N-GFP was a gift from Markku Varjosalo (Addgene plasmid # 172974 ; http://n2t.net/addgene:172974 ; RRID:Addgene_172974)

  • For your References section:

    Combined proximity labeling and affinity purification-mass spectrometry workflow for mapping and visualizing protein interaction networks. Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M. Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 10.1038/s41596-020-0365-x. 10.1038/s41596-020-0365-x PubMed 32778839
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