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Addgene

pRMCD119
(Plasmid #172961)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172961 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRMCD77
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Shigella flexneri wzz K31A mutant gene
  • Species
    Shigella flexneri
  • Insert Size (bp)
    1165

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms additional Q12L and I297T mutations do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRMCD119 was a gift from Renato Morona (Addgene plasmid # 172961 ; http://n2t.net/addgene:172961 ; RRID:Addgene_172961)

  • For your References section:

    Analysis of Shigella flexneri wzz (Rol) function by mutagenesis and cross-linking: wzz is able to oligomerize. Daniels C, Morona R. Mol Microbiol. 1999 Oct;34(1):181-94. doi: 10.1046/j.1365-2958.1999.01591.x. 10.1046/j.1365-2958.1999.01591.x PubMed 10540296
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