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Addgene

pMPRMA53
(Plasmid #172939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE30
  • Backbone size w/o insert (bp) 3400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Shigella flexneri wzz i80 mutant gene
  • Species
    Shigella flexneri
  • Insert Size (bp)
    1162
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMPRMA53 was a gift from Renato Morona (Addgene plasmid # 172939 ; http://n2t.net/addgene:172939 ; RRID:Addgene_172939)

  • For your References section:

    Mutagenesis and chemical cross-linking suggest that Wzz dimer stability and oligomerization affect lipopolysaccharide O-antigen modal chain length control. Papadopoulos M, Morona R. J Bacteriol. 2010 Jul;192(13):3385-93. doi: 10.1128/JB.01134-09. Epub 2010 May 7. 10.1128/JB.01134-09 PubMed 20453100
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