-
PurposeCrowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172933 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5338
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCrowdingSensorGE
-
SpeciesSynthetic
-
Insert Size (bp)1800
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1_crGE was a gift from Arnold Boersma (Addgene plasmid # 172933 ; http://n2t.net/addgene:172933 ; RRID:Addgene_172933) -
For your References section:
A sensor for quantification of macromolecular crowding in living cells. Boersma AJ, Zuhorn IS, Poolman B. Nat Methods. 2015 Mar;12(3):227-9, 1 p following 229. doi: 10.1038/nmeth.3257. Epub 2015 Feb 2. 10.1038/nmeth.3257 PubMed 25643150
