pcDNA3.1_ionRD
(Plasmid
#172931)
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PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172931 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5338
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIonicStrengthSensorRD
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SpeciesSynthetic
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Insert Size (bp)1686
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Linker comprises Arginine and Aspartate as positive/negative amino acids.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_ionRD was a gift from Arnold Boersma (Addgene plasmid # 172931 ; http://n2t.net/addgene:172931 ; RRID:Addgene_172931) -
For your References section:
Ionic Strength Sensing in Living Cells. Liu B, Poolman B, Boersma AJ. ACS Chem Biol. 2017 Oct 20;12(10):2510-2514. doi: 10.1021/acschembio.7b00348. Epub 2017 Sep 6. 10.1021/acschembio.7b00348 PubMed 28853549
