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Addgene

pRSETA_crG18
(Plasmid #172930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172930 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSETA
  • Backbone size w/o insert (bp) 2754
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CrowdingSensorG18
  • Species
    Synthetic
  • Insert Size (bp)
    1614
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSETA_crG18 was a gift from Arnold Boersma (Addgene plasmid # 172930 ; http://n2t.net/addgene:172930 ; RRID:Addgene_172930)

  • For your References section:

    Design and Properties of Genetically Encoded Probes for Sensing Macromolecular Crowding. Liu B, Aberg C, van Eerden FJ, Marrink SJ, Poolman B, Boersma AJ. Biophys J. 2017 May 9;112(9):1929-1939. doi: 10.1016/j.bpj.2017.04.004. 10.1016/j.bpj.2017.04.004 PubMed 28494963
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