pRSETA_crE6G2
(Plasmid #172929)

• Purpose: Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain
• Depositing Lab: Arnold Boersma
• Publication: Liu et al Biophys J. 2017 May 9;112(9):1929-1939. doi: 10.1016/j.bpj.2017.04.004.

Backbone

• Vector backbone: pRSETA
• Backbone size w/o insert (bp): 2754
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CrowdingSensorE6G2
• Species: Synthetic
• Insert Size (bp): 1665
• Mutation: Val317Gly, Met316Gly
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pRSETA_crE6G2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry