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pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10
(Plasmid #172917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172917 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJFRC59
  • Backbone manufacturer
    Gerald Rubin
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    jGCaMP7b-T2A-tdTomato
  • Alt name
    jGCaMP7b-T2A-tdTomato insert
  • Alt name
    variant 1561
  • Species
    Synthetic
  • Insert Size (bp)
    2832
  • Promoter hsp70
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • T2A-tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTACTGAAATCTGCCAA
  • 3′ sequencing primer GGCGTTTACAGCATTT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10 was a gift from GENIE Project (Addgene plasmid # 172917 ; http://n2t.net/addgene:172917 ; RRID:Addgene_172917)
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