pTD55
(Plasmid
#172894)
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PurposemNG^SEC^AID* vector with ccdB markers for cloning homology arms
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 172894 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDD268
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeongreen
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SpeciesBranchiostoma lanceolatum
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mNG^SEC^Degron
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTD55 was a gift from David Reiner (Addgene plasmid # 172894 ; http://n2t.net/addgene:172894 ; RRID:Addgene_172894) -
For your References section:
The Rheb-TORC1 signaling axis functions as a developmental checkpoint. Duong T, Rasmussen NR, Ballato E, Mote FS, Reiner DJ. Development. 2020 Mar 2;147(5). pii: dev.181727. doi: 10.1242/dev.181727. 10.1242/dev.181727 PubMed 32041790