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Addgene

pTD31
(Plasmid #172893)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172893 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDD268
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mNeongreen
  • Species
    Branchiostoma lanceolatum

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mNG^SEC^2xHA ccdB loxP511l

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTD31 was a gift from David Reiner (Addgene plasmid # 172893 ; http://n2t.net/addgene:172893 ; RRID:Addgene_172893)

  • For your References section:

    The Rheb-TORC1 signaling axis functions as a developmental checkpoint. Duong T, Rasmussen NR, Ballato E, Mote FS, Reiner DJ. Development. 2020 Mar 2;147(5). pii: dev.181727. doi: 10.1242/dev.181727. 10.1242/dev.181727 PubMed 32041790
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