pSecEffector
(Plasmid #172891)

• Purpose: Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901
• Depositing Lab: Urartu Seker
• Publication: Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696. doi: 10.1021/acssynbio.9b00062. Epub 2019 Mar 12.

Backbone

• Vector backbone: pZA
• Backbone size w/o insert (bp): 1817
• Total vector size (bp): 10736
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Bxb1
• Insert Size (bp): 1127
• Promoter: nTETO

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: tccctatcagtgatagaga

Gene/Insert 2

• Gene/Insert name: TP901
• Insert Size (bp): 1526
• Promoter: pBAD /AraC

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATGCCATAGCATTTTTATCC

Gene/Insert 3

• Gene/Insert name: TEV Display cassette
• Insert Size (bp): 3298
• Promoter: ProD

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACGTATTAACTTGAAGGCTACGTCC

Gene/Insert 4

• Gene/Insert name: ProD promoter between anti aligned Bxb1 sites
• Insert Size (bp): 262

Cloning Information for Gene/Insert 4

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACGTATTAACTTGAAGGCTACGTCC

Resource Information

• Supplemental Documents:
  • pza-native-teto-bxb1-pbad-tp901-pelb-tev-one-way.gb
• A portion of this plasmid was derived from a plasmid made by: Bxb1 and TP901 amplified from Addgene plasmid #44456

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry