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Addgene

pAG665 MAP4-pFAST
(Plasmid #172865)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172865 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES
  • Backbone size w/o insert (bp) 3969
  • Total vector size (bp) 5655
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAP4-pFAST
  • Alt name
    pFAST fused to MAP4
  • Species
    Synthetic
  • Insert Size (bp)
    1650
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG665 MAP4-pFAST was a gift from Arnaud Gautier (Addgene plasmid # 172865 ; http://n2t.net/addgene:172865 ; RRID:Addgene_172865)
  • For your References section:

    Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging. Benaissa H, Ounoughi K, Aujard I, Fischer E, Goiame R, Nguyen J, Tebo AG, Li C, Le Saux T, Bertolin G, Tramier M, Danglot L, Pietrancosta N, Morin X, Jullien L, Gautier A. Nat Commun. 2021 Nov 30;12(1):6989. doi: 10.1038/s41467-021-27334-0. 10.1038/s41467-021-27334-0 PubMed 34848727