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Addgene

TurboID-mNeongreen-3xFLAG fusion pos3
(Plasmid #172864)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172864 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR™ P2r-P3
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    This plasmid is provided in pre-transformed ccdB survival cells. Other common strains like DH5 alpha or NEB stable cells can also be used.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TurboID
  • Insert Size (bp)
    2199
  • Tags / Fusion Proteins
    • myc (C terminal on insert)
    • mNeongreen (C terminal on insert)
    • FLAG (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TurboID-mNeongreen-3xFLAG fusion pos3 was a gift from Mario de Bono (Addgene plasmid # 172864 ; http://n2t.net/addgene:172864 ; RRID:Addgene_172864)

  • For your References section:

    Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. Artan M, Barratt S, Flynn SM, Begum F, Skehel M, Nicolas A, de Bono M. J Biol Chem. 2021 Sep;297(3):101094. doi: 10.1016/j.jbc.2021.101094. Epub 2021 Aug 18. 10.1016/j.jbc.2021.101094 PubMed 34416233
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