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Addgene

pUC57-Amp_CRISPIE donor B5
(Plasmid #172846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172846 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57-Amp
  • Backbone size w/o insert (bp) 2671
  • Total vector size (bp) 4141
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-Amp_CRISPIE donor B5 was a gift from Haining Zhong (Addgene plasmid # 172846 ; http://n2t.net/addgene:172846 ; RRID:Addgene_172846)

  • For your References section:

    High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion. Zhong H, Ceballos CC, Massengill CI, Muniak MA, Ma L, Qin M, Petrie SK, Mao T. Elife. 2021 Jun 8;10. pii: 64911. doi: 10.7554/eLife.64911. 10.7554/eLife.64911 PubMed 34100715
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