pUC57-Amp_CRISPIE donor B1
(Plasmid
#172842)
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PurposeCRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172842 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57-Amp
- Backbone size w/o insert (bp) 2671
- Total vector size (bp) 3852
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCRISPIE designer exon (phase 0-0) encoding mEGFP
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC57-Amp_CRISPIE donor B1 was a gift from Haining Zhong (Addgene plasmid # 172842 ; http://n2t.net/addgene:172842 ; RRID:Addgene_172842) -
For your References section:
High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion. Zhong H, Ceballos CC, Massengill CI, Muniak MA, Ma L, Qin M, Petrie SK, Mao T. Elife. 2021 Jun 8;10. pii: 64911. doi: 10.7554/eLife.64911. 10.7554/eLife.64911 PubMed 34100715