pRS513
(Plasmid
#17283)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCYB1
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Backbone manufacturerNew England Biolabs
- Backbone size w/o insert (bp) 6846
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTnsB
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SpeciesE. coli transposon Tn7
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Insert Size (bp)2109
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GenBank IDABE77212
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Entrez GenetnsB (a.k.a. R721_28)
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Tag
/ Fusion Protein
- Chitin-binding domain-intein (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer sequence (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS513 was a gift from Nancy Craig (Addgene plasmid # 17283 ; http://n2t.net/addgene:17283 ; RRID:Addgene_17283) -
For your References section:
Formation of a nucleoprotein complex containing Tn7 and its target DNA regulates transposition initiation. Skelding Z, Sarnovsky R, Craig NL. EMBO J. 2002 Jul 1. 21(13):3494-504. 10.1093/emboj/cdf347 PubMed 12093750