pLBM0060
(Plasmid
#172821)
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PurposeLevel 2 module containing HSP21-nYFP HSP21-cYFP OEP7-mTRQ
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAGM4673
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Backbone manufacturerEngler et al. 2014
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][OEP7_mTRQ]
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SpeciesA. thaliana (mustard weed)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLBM0060 was a gift from Benjamin Field (Addgene plasmid # 172821 ; http://n2t.net/addgene:172821 ; RRID:Addgene_172821) -
For your References section:
MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein-protein interactions. Velay F, Soula M, Mehrez M, Belbachir C, D'Alessandro S, Laloi C, Crete P, Field B. Plant Methods. 2022 May 26;18(1):69. doi: 10.1186/s13007-022-00902-1. 10.1186/s13007-022-00902-1 PubMed 35619173