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Addgene

Neurl1-Flag pcDNA3.1
(Plasmid #17282)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17282 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 C-FLAG/Hygro
  • Backbone manufacturer
    Conaway Lab
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neuralized-like
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1725
  • GenBank ID
    BC058386
  • Entrez Gene
    Neurl1a (a.k.a. Nlz, Neu1, Neur1, Neurl, Rnf67, AI450910, AI481072, 2410129E16Rik)
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site SbfI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Neurl1-Flag pcDNA3.1 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 17282 ; http://n2t.net/addgene:17282 ; RRID:Addgene_17282)

  • For your References section:

    Neuralized-like1 targeted to the plasma membrane by N-myristoylation regulates the Notch ligand Jagged1. Koutelou E, Sato S, Tomomori-Sato C, Florens L, Swanson SK, Washburn MP, Kokkinaki M, Conaway RC, Conaway JW, Moschonas NK. J Biol Chem. 2007 Dec 12. ():. 10.1074/jbc.M706974200 PubMed 18077452
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