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Addgene

pLBC0050
(Plasmid #172781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAGM41258
  • Backbone manufacturer
    Engler et al. 2014
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDS 3XFLAG tag N-terminal eYFP (1-174)
  • Species
    Aequorea victoria
  • Mutation
    no

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLBC0050 was a gift from Benjamin Field (Addgene plasmid # 172781 ; http://n2t.net/addgene:172781 ; RRID:Addgene_172781)

  • For your References section:

    MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein-protein interactions. Velay F, Soula M, Mehrez M, Belbachir C, D'Alessandro S, Laloi C, Crete P, Field B. Plant Methods. 2022 May 26;18(1):69. doi: 10.1186/s13007-022-00902-1. 10.1186/s13007-022-00902-1 PubMed 35619173
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