pCS2xVlk1-3xFlag
(Plasmid
#172773)
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PurposemRNA synthesis for injection into embryos
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172773 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVlk1
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SpeciesX. laevis (frog)
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Entrez Genepkdcc.1.S (a.k.a. XELAEV_18041206mg, pkdcc, pkdcc.1, pkdcc1, sgk493, vlk)
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Tag
/ Fusion Protein
- 3xFlag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2xVlk1-3xFlag was a gift from Edward De Robertis (Addgene plasmid # 172773 ; http://n2t.net/addgene:172773 ; RRID:Addgene_172773) -
For your References section:
Genome-wide analysis of dorsal and ventral transcriptomes of the Xenopus laevis gastrula. Ding Y, Colozza G, Zhang K, Moriyama Y, Ploper D, Sosa EA, Benitez MDJ, De Robertis EM. Dev Biol. 2017 Jun 15;426(2):176-187. doi: 10.1016/j.ydbio.2016.02.032. Epub 2016 Mar 23. 10.1016/j.ydbio.2016.02.032 PubMed 27016259