Spike Display_Part 1a Spacer
(Plasmid
#172727)
-
PurposeEncodes Part 1a of HexaPro-D614G spike to be used in the Spike Display system
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 172727 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
* Log in to view industry pricing.
Backbone
-
Vector backboneYTK001
-
Vector typeSynthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSARS-CoV-2 S HexaPro-D614G
-
Alt nameSARS-CoV-2 S 6P-D614G
-
SpeciesSevere acute respiratory syndrome coronavirus 2
-
MutationEctodomain only (AAs 1-1208); 682-685 (furin site) replaced with 'GSAS'; F817P, A892P, A899P, A942P, K986P, V987P, D614G
-
Entrez GeneS (a.k.a. GU280_gp02, spike glycoprotein)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Spike Display_Part 1a Spacer was a gift from IIya Finkelstein (Addgene plasmid # 172727 ; http://n2t.net/addgene:172727 ; RRID:Addgene_172727) -
For your References section:
Rapid characterization of spike variants via mammalian cell surface display. Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ. Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 10.1016/j.molcel.2021.11.024. 10.1016/j.molcel.2021.11.024 PubMed 34919820