pSG5-His-SUMO1
(Plasmid #17271)

• Depositing Lab: Stephen Goff
• Publication: Yueh et al J Virol. 2006 Jan . 80(1):342-52.

Backbone

• Vector backbone: pSG5
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUMO1
• Alt name: Sumo-1
• Species: H. sapiens (human)
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SUM01 originated with Dr. Anne Dejean.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry