pCZGY3195
(Plasmid
#172695)
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PurposePunc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172695 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneGateway final clone
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePunc-25
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Alt nameunc-25p
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Alt nameunc-25 promoter
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SpeciesC. elegans (nematode)
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GenBank ID
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Tag
/ Fusion Protein
- GFP 1-10 (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the Addgene NGS sequence differs from the depositor's annotated sequence. These differences do not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCZGY3195 was a gift from Yishi Jin (Addgene plasmid # 172695 ; http://n2t.net/addgene:172695 ; RRID:Addgene_172695) -
For your References section:
Microtubule-dependent ribosome localization in C. elegans neurons. Noma K, Goncharov A, Ellisman MH, Jin Y. Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. 10.7554/eLife.26376 PubMed 28767038
