-
PurposeEnhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 172667 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV-PE2
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCsy4-PE2
-
SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Unknown
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV-Csy4-PE2 was a gift from Xiaolong Wang (Addgene plasmid # 172667 ; http://n2t.net/addgene:172667 ; RRID:Addgene_172667) -
For your References section:
Enhancing prime editing by Csy4-mediated processing of pegRNA. Liu Y, Yang G, Huang S, Li X, Wang X, Li G, Chi T, Chen Y, Huang X, Wang X. Cell Res. 2021 Jun 8. pii: 10.1038/s41422-021-00520-x. doi: 10.1038/s41422-021-00520-x. 10.1038/s41422-021-00520-x PubMed 34103663