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pcDNA3.3-NL63-D14
(Plasmid #172666)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172666 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.3
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 10000
  • Vector type
    Mammalian Expression, Lentiviral, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HCNV63gp2
  • Alt name
    human NL63 coronavirus Spike protein
  • Species
    human coronavirus NL63
  • Insert Size (bp)
    4000
  • Mutation
    c-terminal deletion of 14aa
  • GenBank ID
    2943499
  • Entrez Gene
    HCNV63gp2 (a.k.a. HCNV63gp2)
  • Promoter CMV
  • Tag / Fusion Protein
    • NA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer TK pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.3-NL63-D14 was a gift from David Nemazee (Addgene plasmid # 172666 ; http://n2t.net/addgene:172666 ; RRID:Addgene_172666)

  • For your References section:

    Broadly neutralizing antibodies target the coronavirus fusion peptide. Dacon C, Tucker C, Peng L, Lee CD, Lin TH, Yuan M, Cong Y, Wang L, Purser L, Williams JK, Pyo CW, Kosik I, Hu Z, Zhao M, Mohan D, Cooper AJR, Peterson M, Skinner J, Dixit S, Kollins E, Huzella L, Perry D, Byrum R, Lembirik S, Drawbaugh D, Eaton B, Zhang Y, Yang ES, Chen M, Leung K, Weinberg RS, Pegu A, Geraghty DE, Davidson E, Douagi I, Moir S, Yewdell JW, Schmaljohn C, Crompton PD, Holbrook MR, Nemazee D, Mascola JR, Wilson IA, Tan J. Science. 2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773. 10.1126/science.abq3773 PubMed 35857439
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