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Addgene

GCEPIA1-SNAPER
(Plasmid #172659)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172659 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unspecified
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    calmodulin
  • Species
    H. sapiens (human)
  • Mutation
    Please see depositor comments

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there is a mixed population of a single base pair in the SNAP tag that could affect the amino acid at position 137. If base is a T, SNAP tag will contain a N137Y mutation. Dep. confirms mutation does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GCEPIA1-SNAPER was a gift from Pingyong Xu (Addgene plasmid # 172659 ; http://n2t.net/addgene:172659 ; RRID:Addgene_172659)

  • For your References section:

    A genetically encoded ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain in the endoplasmic reticulum. Luo C, Wang H, Liu Q, He W, Yuan L, Pingyong Xu P. Biophys Rep (2019) 5, 31–42 10.1007/s41048-019-0082-6
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