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pET-His6-SUMO-MproC145A
(Plasmid #172653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET (https://www.addgene.org/29666/)
  • Backbone manufacturer
    Berkeley Macrolab
  • Backbone size w/o insert (bp) 4786
  • Total vector size (bp) 6010
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Mpro
  • Alt name
    3CLpro
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    1224
  • Mutation
    Catalytic Cysteine 145 mutated to Alanine
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Promoter T7
  • Tags / Fusion Proteins
    • SUMO (N terminal on insert)
    • 6xHis Tag (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original Mpro gene that was mutated for this construct was received from from Linlin Zhang, University of Lubeck (https://science.sciencemag.org/content/368/6489/409)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SUMO fusion can be removed by cleavage with Ulp1 protease

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-His6-SUMO-MproC145A was a gift from Stephen Harrison (Addgene plasmid # 172653 ; http://n2t.net/addgene:172653 ; RRID:Addgene_172653)

  • For your References section:

    Recognition of Divergent Viral Substrates by the SARS-CoV-2 Main Protease. MacDonald EA, Frey G, Namchuk MN, Harrison SC, Hinshaw SM, Windsor IW. ACS Infect Dis. 2021 Sep 10;7(9):2591-2595. doi: 10.1021/acsinfecdis.1c00237. Epub 2021 Aug 26. 10.1021/acsinfecdis.1c00237 PubMed 34437808
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