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pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter
(Plasmid #172599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 172599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10375
  • Vector type
    Mammalian Expression ; EGFP-PEST_Reporter
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myc-PDGFRA-D842V+R914W
  • Alt name
    Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
  • Alt name
    PDGFRA
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    3324
  • Mutation
    PDGFRA-D842V+R914W
  • Entrez Gene
    PDGFRA (a.k.a. CD140A, PDGFR-2, PDGFR2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Extracellular c-myc epitope tag (N terminal on insert)
    • Signal peptide from influenza HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter was a gift from Daniel Kiss (Addgene plasmid # 172599 ; http://n2t.net/addgene:172599 ; RRID:Addgene_172599)

  • For your References section:

    Polygenic risk impacts PDGFRA mutation penetrance in non-syndromic cleft lip and palate. Yu Y, Alvarado R, Petty LE, Bohlender RJ, Shaw DM, Below JE, Bejar N, Ruiz OE, Tandon B, Eisenhoffer GT, Kiss DL, Huff CD, Letra A, Hecht JT. Hum Mol Genet. 2022 Jul 21;31(14):2348-2357. doi: 10.1093/hmg/ddac037. 10.1093/hmg/ddac037 PubMed 35147171
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